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Registro Completo |
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Biblioteca(s): |
Embrapa Soja; Embrapa Unidades Centrais. |
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Data corrente: |
06/07/2022 |
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Data da última atualização: |
26/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARANHATO, A. L. H.; ANGELOTTI-MENDONÇA, J.; MERTZ-HENNING, L. M.; MARIN, S. R. R.; MELO, C. L. P. de; FOLONI, J. S. S.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. |
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Afiliação: |
ANDRÉ LUÍS HARTMANN CARANHATO, Universidade Estadual de Londrina; JÉSSIKA ANGELOTTI-MENDONÇA, CNPSO; LILIANE MARCIA MERTZ HENNING, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; CARLOS LASARO PEREIRA DE MELO, CNPSO; JOSE SALVADOR SIMONETTO FOLONI, CNPSO; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Drought tolerance of elite soybean cultivars with the introgression of transgene AtAREB1. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 57, e02656, 2022. |
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DOI: |
10.1590/S1678-3921.pab2022.v57.02656 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Tolerância à seca em cultivares-elite de soja com a introgressão do transgene AtAREB1 |
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Conteúdo: |
ABSTRACT - The objective of this work was to verify if the introgression of the AtAREB1 gene in the 'LS93-0375' and 'BMX Desafio RR' elite soybean germplasms increases the tolerance of these plants to water deficit. The F4 progenies of these two elite cultivars and of the AtAREB1 transgenic line (BR16-AtAREB1) and its background ('BR16') were subjected to water deficit assays. The water deficit bioassays were performed in a greenhouse using the following six soybean lines: the genetically modified BR16-AtAREB1 and its background 'BR16'; 'LS93' and its F4 progeny, LS93-AtAREB1; and 'BMX Desafio RR' and its F4 progeny, Desafio-AtAREB1. A randomized complete block experimental design was carried out in a 6x2 factorial arrangement, with the six soybean genotypes and two water conditions ? control (C) and water deficit (WD) treatments ? with nine replicates. Soybean genotypes containing the AtAREB1 gene showed better physiological performances under drought stress and altered expressions of drought-responsive genes. The intogression of AtAREB1 in soybean increases the plant drought tolerance, regardless of the genetic background in which the gene was introduced. RESUMO - O objetivo deste trabalho foi verificar se a introgressão do gene AtAREB1 em dois germoplasmas-elite de soja, 'LS93-0375' e 'BMX Desafio RR', aumenta a tolerância dessas plantas ao deficit hídrico. As progênies F4 das duas cultivares-elite e da linhagem transgênica AtAREB1 (BR16- AtAREB1) e de seu background ('BR16') foram submetidas a deficit hídrico. Os bioensaios de deficit hídrico foram realizados em casa de vegetação, tendo-se utilizado as seis seguintes linhagens de soja: a geneticamente modificada BR16-AtAREB1 e seu background 'BR16'; 'LS93' e sua progênie F4, LS93-AtAREB1; e 'BMX Desafio RR' e sua progênie F4, Desafio-AtAREB1. Utilizou-se delineamento experimental de blocos completos com tratamentos casualizados, em arranjo fatorial 6x2, com os seis genótipos de soja e duas condições hídricas ? controle (C) e tratamentos de deficit hídrico (WD) ?, com nove repetições. Os genótipos de soja que contêm o gene AtAREB1 exibiram melhor desempenho fisiológico sob estresse hídrico e expressão alterada de genes responsivos à seca. A introgressão de AtAREB1 na soja aumenta a tolerância à seca, independentemente do background genético em que o gene foi introduzido. MenosABSTRACT - The objective of this work was to verify if the introgression of the AtAREB1 gene in the 'LS93-0375' and 'BMX Desafio RR' elite soybean germplasms increases the tolerance of these plants to water deficit. The F4 progenies of these two elite cultivars and of the AtAREB1 transgenic line (BR16-AtAREB1) and its background ('BR16') were subjected to water deficit assays. The water deficit bioassays were performed in a greenhouse using the following six soybean lines: the genetically modified BR16-AtAREB1 and its background 'BR16'; 'LS93' and its F4 progeny, LS93-AtAREB1; and 'BMX Desafio RR' and its F4 progeny, Desafio-AtAREB1. A randomized complete block experimental design was carried out in a 6x2 factorial arrangement, with the six soybean genotypes and two water conditions ? control (C) and water deficit (WD) treatments ? with nine replicates. Soybean genotypes containing the AtAREB1 gene showed better physiological performances under drought stress and altered expressions of drought-responsive genes. The intogression of AtAREB1 in soybean increases the plant drought tolerance, regardless of the genetic background in which the gene was introduced. RESUMO - O objetivo deste trabalho foi verificar se a introgressão do gene AtAREB1 em dois germoplasmas-elite de soja, 'LS93-0375' e 'BMX Desafio RR', aumenta a tolerância dessas plantas ao deficit hídrico. As progênies F4 das duas cultivares-elite e da linhagem transgênica AtAREB1 (BR16- AtAREB1) e de seu background ('BR... Mostrar Tudo |
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Thesagro: |
Gene; Germoplasma; Glycine Max; Linhagem; Progênie; Soja. |
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Thesaurus Nal: |
Drought tolerance; Genetic background; Genotype; Germplasm; Hybridization; Introgression; Soybeans; Transgenes. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144496/1/Drought-tolerance-elite-2022.pdf
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Marc: |
LEADER 03629naa a2200409 a 4500
001 2145791
005 2022-08-26
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1590/S1678-3921.pab2022.v57.02656$2DOI
100 1 $aCARANHATO, A. L. H.
245 $aDrought tolerance of elite soybean cultivars with the introgression of transgene AtAREB1.$h[electronic resource]
260 $c2022
500 $aTítulo em português: Tolerância à seca em cultivares-elite de soja com a introgressão do transgene AtAREB1
520 $aABSTRACT - The objective of this work was to verify if the introgression of the AtAREB1 gene in the 'LS93-0375' and 'BMX Desafio RR' elite soybean germplasms increases the tolerance of these plants to water deficit. The F4 progenies of these two elite cultivars and of the AtAREB1 transgenic line (BR16-AtAREB1) and its background ('BR16') were subjected to water deficit assays. The water deficit bioassays were performed in a greenhouse using the following six soybean lines: the genetically modified BR16-AtAREB1 and its background 'BR16'; 'LS93' and its F4 progeny, LS93-AtAREB1; and 'BMX Desafio RR' and its F4 progeny, Desafio-AtAREB1. A randomized complete block experimental design was carried out in a 6x2 factorial arrangement, with the six soybean genotypes and two water conditions ? control (C) and water deficit (WD) treatments ? with nine replicates. Soybean genotypes containing the AtAREB1 gene showed better physiological performances under drought stress and altered expressions of drought-responsive genes. The intogression of AtAREB1 in soybean increases the plant drought tolerance, regardless of the genetic background in which the gene was introduced. RESUMO - O objetivo deste trabalho foi verificar se a introgressão do gene AtAREB1 em dois germoplasmas-elite de soja, 'LS93-0375' e 'BMX Desafio RR', aumenta a tolerância dessas plantas ao deficit hídrico. As progênies F4 das duas cultivares-elite e da linhagem transgênica AtAREB1 (BR16- AtAREB1) e de seu background ('BR16') foram submetidas a deficit hídrico. Os bioensaios de deficit hídrico foram realizados em casa de vegetação, tendo-se utilizado as seis seguintes linhagens de soja: a geneticamente modificada BR16-AtAREB1 e seu background 'BR16'; 'LS93' e sua progênie F4, LS93-AtAREB1; e 'BMX Desafio RR' e sua progênie F4, Desafio-AtAREB1. Utilizou-se delineamento experimental de blocos completos com tratamentos casualizados, em arranjo fatorial 6x2, com os seis genótipos de soja e duas condições hídricas ? controle (C) e tratamentos de deficit hídrico (WD) ?, com nove repetições. Os genótipos de soja que contêm o gene AtAREB1 exibiram melhor desempenho fisiológico sob estresse hídrico e expressão alterada de genes responsivos à seca. A introgressão de AtAREB1 na soja aumenta a tolerância à seca, independentemente do background genético em que o gene foi introduzido.
650 $aDrought tolerance
650 $aGenetic background
650 $aGenotype
650 $aGermplasm
650 $aHybridization
650 $aIntrogression
650 $aSoybeans
650 $aTransgenes
650 $aGene
650 $aGermoplasma
650 $aGlycine Max
650 $aLinhagem
650 $aProgênie
650 $aSoja
700 1 $aANGELOTTI-MENDONÇA, J.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aMARIN, S. R. R.
700 1 $aMELO, C. L. P. de
700 1 $aFOLONI, J. S. S.
700 1 $aNEUMAIER, N.
700 1 $aFARIAS, J. R. B.
700 1 $aNEPOMUCENO, A. L.
773 $tPesquisa Agropecuária Brasileira$gv. 57, e02656, 2022.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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Data corrente: |
07/04/2015 |
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Data da última atualização: |
08/04/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
KICH, J. D.; UTHE, J. J.; BENAVIDES, M. V.; CANTAO, M. E.; ZANELLA, R.; TUGGLE, C. K.; BEARSON, S. M. D. |
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Afiliação: |
JALUSA DEON KICH, CNPSA; JOLITE JANUTENAITE UTHE, Iowa State University; MAGDA VIEIRA BENAVIDES, SRI; MAURICIO EGIDIO CANTAO, CNPSA; RICARDO ZANELLA; CHRISTOPHER KEITH TUGGLE, Iowa State University; SHAWN MICHELLE DUNKIN BEARSON, Iowa State University. |
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Título: |
TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Applied Genetics, v. 55, n. 2, p. 267-71, 2014. |
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DOI: |
10.1007/s13353-014-0199-8 |
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Idioma: |
Inglês |
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Conteúdo: |
oll-like receptor 4 (TLR4) is a key factor in the innate immune recognition of lipopolysaccharide (LPS) from Gram-negative bacteria. Previous studies from our group identified differences in the expression profile of TLR4 and genes affected by the TLR4 signaling pathway among pigs that shed varying levels of Salmonella, a Gram-negative bacterium. Therefore, genetic variation in this gene may be involved with the host's immune response to bacterial infections. The current study screened for single nucleotide polymorphisms (SNPs) in the TLR4 gene and tested their association with Salmonella fecal shedding. Pigs (n = 117) were intranasally challenged at 7 weeks of age with 1 × 10(9) CFU of S. Typhimurium χ4232 and were classified as low or persistent Salmonella shedders based on the levels of Salmonella being excreted in fecal material. Salmonella fecal shedding was determined by quantitative bacteriology on days 2, 7, 14, and 20/21 post exposure, and the cumulative levels of Salmonella were calculated to identify the low (n = 20) and persistent (n = 20) Salmonella shedder pigs. From those 40 animals, the TLR4 region was sequenced, and 18 single nucleotide polymorphisms (SNPs) in TLR4 were identified. Twelve SNPs have been previously described and six are novel SNPs of which five are in the 5' untranslated region and one is in intron 2. Single marker association test identified 13 SNPs associated with the qualitative trait of Salmonella fecal shedding, and seven of those SNPs were also associated with a quantitative measurement of fecal shedding (P < 0.05). Using a stepwise regression process, a haplotype composed of SNPs rs80787918 and rs80907449 (P ≤ 4.0 × 10(-3)) spanning a region of 4.9 Kb was identified, thereby providing additional information of the influence of those SNPs on Salmonella fecal shedding in pigs. Menosoll-like receptor 4 (TLR4) is a key factor in the innate immune recognition of lipopolysaccharide (LPS) from Gram-negative bacteria. Previous studies from our group identified differences in the expression profile of TLR4 and genes affected by the TLR4 signaling pathway among pigs that shed varying levels of Salmonella, a Gram-negative bacterium. Therefore, genetic variation in this gene may be involved with the host's immune response to bacterial infections. The current study screened for single nucleotide polymorphisms (SNPs) in the TLR4 gene and tested their association with Salmonella fecal shedding. Pigs (n = 117) were intranasally challenged at 7 weeks of age with 1 × 10(9) CFU of S. Typhimurium χ4232 and were classified as low or persistent Salmonella shedders based on the levels of Salmonella being excreted in fecal material. Salmonella fecal shedding was determined by quantitative bacteriology on days 2, 7, 14, and 20/21 post exposure, and the cumulative levels of Salmonella were calculated to identify the low (n = 20) and persistent (n = 20) Salmonella shedder pigs. From those 40 animals, the TLR4 region was sequenced, and 18 single nucleotide polymorphisms (SNPs) in TLR4 were identified. Twelve SNPs have been previously described and six are novel SNPs of which five are in the 5' untranslated region and one is in intron 2. Single marker association test identified 13 SNPs associated with the qualitative trait of... Mostrar Tudo |
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Thesagro: |
Genética animal; Suíno. |
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Thesaurus NAL: |
Animal genetics; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02675naa a2200253 a 4500
001 2012931
005 2015-04-08
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s13353-014-0199-8$2DOI
100 1 $aKICH, J. D.
245 $aTLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs.$h[electronic resource]
260 $c2014
520 $aoll-like receptor 4 (TLR4) is a key factor in the innate immune recognition of lipopolysaccharide (LPS) from Gram-negative bacteria. Previous studies from our group identified differences in the expression profile of TLR4 and genes affected by the TLR4 signaling pathway among pigs that shed varying levels of Salmonella, a Gram-negative bacterium. Therefore, genetic variation in this gene may be involved with the host's immune response to bacterial infections. The current study screened for single nucleotide polymorphisms (SNPs) in the TLR4 gene and tested their association with Salmonella fecal shedding. Pigs (n = 117) were intranasally challenged at 7 weeks of age with 1 × 10(9) CFU of S. Typhimurium χ4232 and were classified as low or persistent Salmonella shedders based on the levels of Salmonella being excreted in fecal material. Salmonella fecal shedding was determined by quantitative bacteriology on days 2, 7, 14, and 20/21 post exposure, and the cumulative levels of Salmonella were calculated to identify the low (n = 20) and persistent (n = 20) Salmonella shedder pigs. From those 40 animals, the TLR4 region was sequenced, and 18 single nucleotide polymorphisms (SNPs) in TLR4 were identified. Twelve SNPs have been previously described and six are novel SNPs of which five are in the 5' untranslated region and one is in intron 2. Single marker association test identified 13 SNPs associated with the qualitative trait of Salmonella fecal shedding, and seven of those SNPs were also associated with a quantitative measurement of fecal shedding (P < 0.05). Using a stepwise regression process, a haplotype composed of SNPs rs80787918 and rs80907449 (P ≤ 4.0 × 10(-3)) spanning a region of 4.9 Kb was identified, thereby providing additional information of the influence of those SNPs on Salmonella fecal shedding in pigs.
650 $aAnimal genetics
650 $aSwine
650 $aGenética animal
650 $aSuíno
700 1 $aUTHE, J. J.
700 1 $aBENAVIDES, M. V.
700 1 $aCANTAO, M. E.
700 1 $aZANELLA, R.
700 1 $aTUGGLE, C. K.
700 1 $aBEARSON, S. M. D.
773 $tJournal of Applied Genetics$gv. 55, n. 2, p. 267-71, 2014.
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